TITLE	The relationships of cultivated soybeans and their wild relatives collected from Taiwan: revealed by seed proteins
AUTHOR	Yue-Ie C. Hsing Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China Kim-Leong Hsieh Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China Yih-Ching Huang Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China Jaw-Shu Hsieh Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China
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ABSTRACT	We conducted molecular studies of the taxonomic relationships between cultivated soybean, Glycine max, and its wild relatives, G. soja, G. tomentella, and G. tabacina, collected in Taiwan and the nearby islands. The G. max and G. soja are annual and the others are perennial. We used protein gels and Western blot to study their seed proteins¡Xincluding two major storage proteins, lectin, and several seed maturation proteins. We performed RFLP analysis using soybean seed maturation protein cDNA clones as the probes. The data were used to assess the degree of genetic variability and similarity existing between and within different species of Glycine collected in Taiwan. The results confirm and extend the present model of the taxonomic relationships. Seed maturation proteins may serve as a marker that reveals relationships between the member species of the Glycine taxon.
KEYWORD	Coefficients of genetic similarity; Glycine species; Polyacrylamide gel electrophoresis; Restriction fragment length polymorphism; Soybean seed lectin; Soybean seed maturation proteins; Soybean seed storage proteins; Western blot;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 36 Number 2 April 1995, page 65-72, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China