| TITLE |
Quantifying termination of transcription by spinach chloroplast RNA polymerase using supercoiled templates containing tandem copies of the thra terminator |
| AUTHOR |
Liang-Jwu Chen
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China |
| FULL TEXT |
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| ABSTRACT |
A method was developed to quantify the efficiency with which a transcription terminator of the E. coli threonine attenuator, thra, is recognized by spinach chloroplast RNA polymerase. Multiple tandem copies of the thra terminator were cloned downstream from a strong chloroplast promoter. These supercoiled DNAs were transcribed in vitro and the termination efficiency determined by quantifying the relative amount of transcripts that ended at each successive terminator. A simple calculation showed that about 63% of the RNAs made by spinach chloroplast or T7 RNA polymerase were terminated at each thra terminator. |
| KEYWORD |
Chloroplast RNA polymerase; Transcription terminator; |
| ARTICLE INFO |
Botanical Bulletin of Academia Sinica, Volume 36 Number 2 April 1995, page 95-100, 6 pages |
| PUBLISHER |
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China |
