TITLE	Grana stacking is normal in a chlorophyll-deficient LT8 mutant of rice
AUTHOR	Chi-Ming Yang Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115, Republic of China Hsiu-Yu Chen Department of Agronomy, National Chung-Hsing University, Taichung, Taiwan 402, Republic of China
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ABSTRACT	Thylakoid proteins and chloroplasts isolated from chlorophyll-deficient rice mutant LT8 and its Norin 8 parent type (Oryza sativa) were compared using SDS-PAGE and electron microscopy. The LT8 mutant contained approximately 37% of the amount of chlorophyll found in the Norin 8; the ratio of chlorophyll a/b for LT8 and Norin 8 were 6.4 and 3.2, respectively. SDS-PAGE profiles showed that LT8 mutant contained approximately 10% of the amount of LHCII polypeptide found in the Norin 8, although both biotypes produced similar levels of LHCI and other thylakoid proteins. Electron microscopy showed that the grana stacks were similar in the LT8 mutant and Norin 8, suggesting that LHCII is not the only factor regulating the grana stacking of thylakoid membrane.
KEYWORD	Chlorophyll-deficient mutant; Grana stacking; LHCII; Oryza sativa;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 37 Number 1 January 1996, page 31-34, 4 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China