TITLE	Use of random amplified polymorphic DNA markers for the detection of genetic variation in Phytophthora cinnamomi in Taiwan
AUTHOR	T. T. Chang Division of Forest Protection, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, Taiwan, Republic of China W. W. Yang Division of Forest Protection, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, Taiwan, Republic of China W. Y. Wang Division of Forest Protection, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei, Taiwan, Republic of China
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ABSTRACT	Taiwanese isolates of Phytophthora cinnamomi were examined for genetic diversity by the random amplified polymorphic DNA assay. The amount of genetic variation was evaluated by polymerase chain reaction amplification with a set of nine random 10-mer primers. All amplifications revealed scorable polymorphisms among the isolates, and a total of 217 band positions was scored for the nine primers tested. Genetic distances between each of the isolates were calculated and cluster analysis was used to generate a dendrogram showing relationships between them. Isolates did not cluster into groups corresponding to their mating types. Primer OPS-13 did produce one 2.7 kb band for all isolates of A1 mating type, but not for A2 isolates. Moreover, primer OPE-06 amplified one 1.3 kb band for all A1 mating type isolates, while that was absent in A2 isolates except for three isolates obtained from avocado roots. Genetic differentiation of ten isolates obtained from the same location between two mating types was significantly high, indicating that no hybridization occurred between A1 and A2 mating types in that situation. Primer OPE-4 amplified a 680 bp fragment which was only observed in three isolates obtained from avocado roots. Also, genetic differentiation between isolates from avocado and those from other sources was significantly strong (distance between 0.262 and 0.412). These results suggest host specified races might occur in P. cinnamomi and the RAPD technique could be used to differentiate races within P. cinnamomi.
KEYWORD	Phytophthora cinnamomi; RAPD; Taiwan;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 37 Number 3 July 1996, page 165-171, 7 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China