TITLE	Effect of naphthaleneacetic acid on endogenous indole-3-acetic acid, peroxidase and auxin oxidase in hypocotyl cuttings of soybean during root formation
AUTHOR	Zin-Huang Liu Department of Biology, Institute of Life Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan 804, Republic of China I-Chao Hsiao Department of Biology, Tunghai University, Taichung, Taiwan, Republic of China Ya-Wen Pan Department of Biology, Tunghai University, Taichung, Taiwan, Republic of China
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ABSTRACT	The effect of exogenous naphthaleneacetic acid (NAA) on the internal levels of indole-3-acetic acid (IAA) in rooting hypocotyls of Glycine max was studied. The hypocotyls of NAA-treated cuttings grew significantly higher numbers of adventitious roots with an increase in endogenous IAA levels that corresponded with a decrease in IAA oxidase activity (32 kDa) examined. Moreover, a decline of peroxidase activity was accompanied by a decrease of lignin content during root formation. Caffeic acid and ferulic acid, two critical phenolic compounds for lignin synthesis, accumulated in NAA-treated tissues. Consequently, the increased IAA levels with a decrease of IAA oxidase activity accompanied a lower lignin content and a reduced peroxidase activity in NAA-treated tissues suggests that the induction of adventitious roots by NAA in soybean cuttings may be due to to the higher IAA levels accumulated in tissues.
KEYWORD	Adventitious rooting; Indole-3-acetic acid; IAA oxidase; Naphthaleneacetic acid; Peroxidase; Glycine max;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 37 Number 4 October 1996, page 247-253, 7 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China