TITLE Isolation of cDNA clones for genes that are specifically expressed in the rice embryo
AUTHOR Peng-Wen Chen
Institute of Botany, National Chung Hsing University, Taichung, Taiwan 40227, Republic of China
Li-Wen Fang
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China
Juey-Wen Lin
Agricultural Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan, Republic of China
Hsin-Sheng Tsay
Department of Agronomy, Taiwan Agricultural Research Institute, Taichung, Taiwan, Republic of China
Hsin-Kan Wu
Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China
Liang-Jwu Chen
Institute of Botany, National Chung Hsing University, Taichung, Taiwan 40227, Republic of China
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ABSTRACT We isolated thirty-eight cDNA clones representing genes expressed in the rice embryo by differential screening of a cDNA library made with poly(A) RNA from embryos at the stage of 10-days after pollination. Eighteen cDNA clones displayed either a significant nucleic acid or amino acid sequence similarity to sequences known in the GenBank, i.e., two rice oleosins, four globulin or glutelin-like proteins, two ABA response proteins, two LEA-like proteins, one trypsin inhibitor, one metallothionein-like protein, one protein kinase, one leucine-zipper containing protein, one proline-rich protein, and three genes of unknown function. Experimental results indicated the presence of redundant cDNA clones for genes that produced an abundant amount of mRNAs in the embryo. RNA slot blot hybridization demonstrated the embryo-specific expression for thirty-seven cDNA clones. The majority of the identified cDNAs had not been known in rice. Results in this study contribute toward the on-going attempt to isolate embryo-specific and novel genes in rice.
KEYWORD cDNA library; Differential screening; Embryogenesis; Rice (Oryza sativa);
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 38 Number 1 January 1997, page 13-20, 8 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China