TITLE Genetic polymorphism of seven populations of Capsella bursa-pastoris based on RAPD markers
AUTHOR Yau-Wen Yang
Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China
Warren H.J. Kuo
Department of Agronomy, National Taiwan University, Taiwan 106, Republic of China
Teck Hiang Wong
Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China
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ABSTRACT Capsella bursa-pastoris is considered to be a potential vegetable crop. Together with two accessions of mustard (Brassica juncea) as outgroups, seven populations of C. bursa-postoris were used to study genetic polymorphism using random amplified polymorphic DNA (RAPD) markers. Using 12 primers, a total of 158 RAPD markers were selected among these populations. Among them, 91 are either specific for C. bursa-postris or present in both C. bursa-postoris and B. juncea. In Capsella populations, 40 RAPD markers are polymorphic, 5 markers are population specific, and 46 markers are monomorphic with variation of banding density. Each RAPD marker was treated as either having unweighted character or weighted character. Using UPGMA for cluster analysis, the polymorphic tree structures based on unweighted and weighted character have a similar phylogenetic relationship among these tested populations. With one exception, the phylogenetic relationship of Taiwan origin is correlated with the geographical distribution of these populations. These results may provide us information of genetic variation to be used in a varietal development program and to find specific markers linked to important traits.
KEYWORD Capsella bursa-pastrois; Genetic polymorphism; RAPDs;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 39 Number 1 January 1998, page 17-21, 5 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China