TITLE Determination of genetic stability of micropropagated plants of ginger using Random Amplified Polymorphic DNA (RAPD) markers
AUTHOR G.R. Rout
Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar - 751 015, Orissa, India
P. Das
Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar - 751 015, Orissa, India
S. Goel
Plant Cytogenetics and Molecular Biology Laboratory, Department of Botany, Delhi University, Delhi - 110 007, India
S.N. Raina
Plant Cytogenetics and Molecular Biology Laboratory, Department of Botany, Delhi University, Delhi - 110 007, India
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ABSTRACT Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of micropropagated plants of Zingiber officinales cv. V3S18. Fifteen arbitrary decamers were used to amplify DNA from in vivo and in vitro plant material to assess the genetic fidelity. All RAPD profiles from micropropagated plants were monomorphic and similar to those of field grown control plants. No variation was detected within the micropropagated plants. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the systems of micropropagation is discussed.
KEYWORD Genetic markers; Genetic stability; Micropropagation; RAPD;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 39 Number 1 January 1998, page 23-27, 5 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China