TITLE Complete nucleotide sequence of the intergenic spacer between 25S and 17S rDNA in Miscanthus sinensis var. glaber
AUTHOR Tzen-Yuh Chiang
Department of Biology, National Cheng-Kung University, Tainan 700, Taiwan
Yu-Chung Chiang
Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan
Chang-Hung Chou
Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan
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ABSTRACT The nucleotide sequence of the intergenic spacer (IGS) between the 25S and 17S ribosomal RNA genes of Miscanthus sinensis var. glaber was determined. The spacer length (2,178 bp) in Miscanthus is close to that of rice IGS (2,140 bp). Many repeated elements with consensus lengths of 34 and 59 base pairs are found in the nontranscribed region (NTS), upstream of the putative transcription initiation site (TIS). Subrepeats B with four copies are longer and better conserved than subrepeats A with ten copies. The sequences of the external transcribed region (ETS) show high identity, ranging from 66% to 76%, among Miscanthus, rice, maize, and wheat.
KEYWORD Intergenic spacer (IGS); Miscanthus sinensis var. glaber; rDNA gene; Repeated elements; Transcription initiation site;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 39 Number 4 October 1998, page 241-244, 4 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China