TITLE Protease and amylase production of Streptomyces rimosus in submerged and solid state cultivations
AUTHOR Shang-Shyng Yang
Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan
Jan-Yi Wang
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ABSTRACT The protease activity of Streptomyces rimosus TM-55 was first detected after 12 h of growth in submerged cultivation, and this activity peaked after 166 h of incubation. In solid state cultivation, protease was first secreted at 24 h, with the secretion peaking at 232 h. Amylase activity could be detected after 6 h in submerged cultivation, and it afterwards peaked after 48 h of incubation. In solid state cultivation, it began to be secreted at 24 h, and this secretion peaked after 180 h. Each gram of starch yielded 17.4 and 691.3 units of protease and amylase in submerged cultivation, respectively; whereas the values were 26.7 and 2,642.7 units in solid state cultivation. a-Amylase was the major amylase in both cultivation methods, and glucoamylase and debranching activity were minor components. Protease and amylase produced with both cultivation methods had a similar optimal pH, between 6.0 and 7.0, and optimal temperature, between 35 and 45°C. The enzyme activities produced in solid state cultivation were more stable with pH and temperature changes than those produced in submerged cultivation.
KEYWORD Amylase; Protease; Streptomyces; Submerged and solid state cultivations;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 40 Number 4 October 1999, page 259-265, 7 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China