TITLE	Expression of rice prolamin RP3 promoter exhibits a positive association with cellular pigment contents in transgenic tobacco suspension cultures
AUTHOR	Hsien-Jung Chen Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China Chih-Yuan Yang Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China Yean-Ching Chen Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China Ching-San Chen Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529, Republic of China
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ABSTRACT	Three transgenic tobacco regenerants (lines RP3/2, RP3/7 and RP3/18) harboring rice RP3/GUS chimeric gene inserts in their genomes were isolated previously and used to establish cell suspension cultures (Yang et al., 2000). A differential GUS expression pattern together with morphological, biochemical, and molecular variations were observed among these cell lines (Chen et al., 1999). In this report we used pigments such as carotenoids and chlorophylls a and b as markers to study the possible relationship between RP3 promoter activity and cellular pigment contents, and a positive association was found among these cell lines. RP3/2, which contained the highest cellular pigment contents, also exhibited the highest GUS expression level. The RP3 promoter activity in RP3/2 continuously decreased and was parallel with the reduction of pigment contents within a 12-day growth period after subculture. In RP3/18, both also showed a parallel association and remained relatively constant during cell growth. Based on these results, a positive association between RP3 promoter activity and cellular pigment content was found among and within cell lines. The meaning of the association and its possible explanation are discussed.
KEYWORD	Pigment; Prolamin; Rice; RP3 promoter; Tobacco;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 2 April 2000, page 93-98, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China