TITLE RFLP and inheritance patterns of chloroplast DNA in intergeneric hybrids of Phalaenopsis and Doritis
AUTHOR Song-Bin Chang
Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China
Wen-Huei Chen
Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China
Hong-Hwa Chen
Department of Biology, and 3Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan 701, Republic of China
Yang-Ming Fu
Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China
Yih-Shyan Lin
Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China
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ABSTRACT The mode of inheritance of chloroplasts was analyzed using restriction fragment length polymorphism (RFLP) in both interspecific hybrids of Phalaenopsis and intergeneric hybrids of Phalaenopsis and Doritis. Chloroplast DNA digested with Dra I followed by hybridization with an rbcL probe revealed that Phalaenopsis amabilis, Phalaenopsis aphrodite, and Phalaenopsis stuartiana, which belong to the taxonomic section PHALAENOPSIS, have the same size 2.0-kb fragment. Both Phalaenopsis mannii and Phalaenopsis amboinensis have a 2.3-kb fragment, while Doritis pulcherrima has a 3.5-kb fragment. In both interspecific and intergeneric hybrids, maternal inheritance of the chloroplast genome was detected. The hybrids of both reciprocal crosses (A x B and B x A) are registered with the same hybrid names in Sander's List of Orchid Hybrids at the Royal Horticultural Society, despite harboring chloroplast DNA from different parents. These results suggest that the chloroplast DNA can be used as a marker for identification of parentship and for phylogenetic studies of taxonomy.
KEYWORD cpDNA inheritance pattern; Doritis; Phalaenopsis; RFLP;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 41 Number 3 July 2000, page 219-223, 5 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China