TITLE	Genetic analysis of Chrysanthemum hybrids based on RAPD molecular markers
AUTHOR	Sheng Chung Huang Taichung District Agricultural Improvement Station, Changhua, Taiwan, Republic of China Chi Chu Tsai Taichung District Agricultural Improvement Station, Changhua, Taiwan, Republic of China Chian Shinn Sheu Taichung District Agricultural Improvement Station, Changhua, Taiwan, Republic of China
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ABSTRACT	Forty-five random primers were screened, of which twenty-two primers were selected to detect the molecular marker in three hybrid combinations of Chrysanthemum by using random amplified polymorphic DNA (RAPD). From this study, the patterns of molecular markers could be classified into seven types: Type I markers shared bands in both parents, and offspring; Type II markers shared bands in male and female parents; Type III markers shared bands in male parent and offspring; Type IV markers shared bands in female parent and offspring; Type V markers were presented in the male parent only; Type VI markers were present in the female parent only; Type VII markers were present in offspring only. Of these, only Type III markers were suitable for identifying the true male parent. Different unique markers of Type VII in offspring are quite suitable as identifying markers of new hybrids to protect the rights of plant breeders. In this study, 34.4% to 48.9% of the RAPD markers were found to reveal additivity among parents and offspring in three hybrid combinations of Chrysanthemum. However, 38% to 52.6% of markers (Type II, V, and VI) were absent in offspring, but 11.6% to 13.1% of unique markers (Type VII) were present in offspring. Moreover, there were no definite rules as to whether markers in offspring were more similar to female or to male parents by similarity analysis. In two hybrid combinations, the parents were more similar to each other than either was to the offspring. The above results illustrate that the genetics of Chrysanthemum are very complex. RAPDs, however, are a powerful tool to detect different molecular markers in hybrid populations of Chrysanthemum cultivars.
KEYWORD	Chrysanthemum; Hybrids; Molecular marker; RAPD;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 4 October 2000, page 257-262, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China