TITLE	Isolation and characterization of the third gene encoding a 16.9 kDa class I low-molecular-mass heat shock protein, Oshsp16.9C, in rice
AUTHOR	Pi-Fang Linda Chang Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan 402, Republic of China Chung-Yi Huang Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Fa-Cheng Chang Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Fa-Cheng Chang Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Tong-Seung Tseng Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Tong-Seung Tseng Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Wan-Chi Lin Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Wan-Chi Lin Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China Chu-Yung Lin Department of Botany, National Taiwan University, Taipei, Taiwan 106, Republic of China
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ABSTRACT	Using rice cDNA pTS1 encoding a 16.9 kDa heat shock protein (HSP) as a probe for Southern hybridization analysis, we observed five prominent bands of 9.5, 5.9, 3.4, 2.5, and 1.7 kb in the Eco RI digests of rice genomic DNA and found them to contain six individual genes. The 5.9 kb DNA fragment was further digested with Hind III to generate three fragments of 3.5, 1.7 and 0.7 kb, and we found, using pTS1 cDNA as a probe, the 3.5 kb fragment contained a putative low-molecular-mass (LMM) HSP gene. The DNA sequencing of 3.5 kb fragment revealed encoding of a presumptive 16.9 kDa HSP (149 amino acid residues) with predicted pI value of 6.42. The nucleotide sequence of this gene was highly homologous to the coding regions of two rice class I LMM HSP genes, Oshsp 16.9A and Oshsp16.9B, sharing 93.1% and 94.3% sequence identity, respectively, as published previously in our laboratory. The deduced amino acid sequence of this gene is similar to those of the Oshsp16.9A and Oshsp16.9B genes with a difference of only 11 and 10 amino acids, respectively. It was hence designated as Oshsp16.9C (accession no. U81385). We used 3' UTRs (untranslated regions) for analysis of Oshsp16.9C gene expression since the 3' UTRs of these three genes showed very low sequence homology.
KEYWORD	Heat shock protein; Heat shock gene; Low-molecular-mass heat shock protein; Oryza sativa L;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 42 Number 2 April 2001, page 85-92, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China