TITLE	Comparative analyses of disease resistant and nonresistant lines from maize x Zea diploperennis by GISH
AUTHOR	Wen-Hui Wei The Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan Hubei 430072, The People's Republic of China Rui Qin The Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan Hubei 430072, The People's Republic of China Yun-Chun Song The Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan Hubei 430072, The People's Republic of China Le-Qun Guo Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, The People's Republic of China Ming-Guang Gu Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, The People's Republic of China
FULL TEXT	[in HTML format] [in PDF format]
ABSTRACT	After a cross between maize inbred line Lu9 and Zea diploperennis (DP) and a backcross between their F1 and Lu9, the BC1 was reproduced with parthenogenesis induced by chemicals. In this study a2-(1) and a2-(6), the two tested, stable alloplasmic sister lines, were obtained through selecting and selfing of the parthenogenetical progenies for over ten generations. The line a2-(1) showed DP characters, such as resistance to Helminthosporium turcium Pass, H. maydis Nisik, and H. carbonum ULLstrup while a2-(6) exhibited no such resistance. The introgressed DP DNA segments were successfully detected, and their physical location on chromosomes were compared in these two lines by genomic in situ hybridization (GISH). In line a2-(1), the hybridization signals were located on the long arms of chromosomes 1, 2 and 8, and on the short arm of chromosome 6; in line a2-(6), they were located only on the long arms of chromosomes 1, 2 and 8, with no signal on chromosome 6 at all. The distribution of introgressed DP segments on chromosomes, reasons for differences of disease resistance between these two lines, and the existence of resistance genes in the introgressed segments are discussed.
KEYWORD	Alien introgressed segments; Disease resistance genes; Genomic in situ hybridization (GISH); Maize; Zea diploperennis;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 42 Number 2 April 2001, page 109-114, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China