TITLE Genetic diversity and relationship among peach cultivars based on Random Amplified Microsatellite Polymorphism (RAMP)
AUTHOR Hsiu-Yu Cheng
Deptartment of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China
Wei-Chen Yang
Deptartment of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China
Ju-Ying Hsiao
Deptartment of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China
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ABSTRACT Peach (Prunus persica (L.) Batsch) is a common fruit tree species, mainly in the temperate region. It is an important economic plant in Taiwan. In the present study, the genetic relationships among 26 cultivars of common peach (P. persica var. vulgaris Maxim.), 12 cultivars of nectarine (P. persica var. nectarina Maxim.), and three cultivars of flat peach (P. persica var. platycarpa Bailey) were estimated using RAMP markers. Eighty-two polymorphic bands were obtained using ten combinations of primers. The cluster analysis based on RAMP data revealed that the groupings were generally consistent with the classification of the varieties and the regions of origin of cultivars. The common peach cultivars originating in China and Japan formed a cluster. A possible explanation is that the Japanese cultivars may be developed from cultivars introduced from China. Within flat peach cultivars, the groupings also indicated that the genetic relationship among cultivars is correlated with the regions of origin of cultivars. Analysis of molecular variance (AMOVA) revealed that the variance components among and within three peach groups expressed as percentages of the total variation were 30.3% and 69.7%, respectively.
KEYWORD Peach; Prunus persica; Genetic relationship; RAMP; AMOVA;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 42 Number 3 July 2001, page 201-206, 6 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China