TITLE	The efficiencies of various embryo rescue methods in interspecific crosses of Lilium
AUTHOR	Hai-Shan Chi Department of Horticulture, National Chiayi University, Chiayi 600, Taiwan, Republic of China
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ABSTRACT	The purpose of this research was to compare the efficiencies of various embryo rescue methods in interspecific hybridization of lily. This research included ten combinations of crosses with six crossing types (Lilium longiflorum X Asiatic hybrid, LA; Oriental hybrid X Asiatic hybrid, OA; Oriental hybrid X Lilium longiflorum, OL; Lilium longiflorum X Oriental hybrid, LO; Asiatic hybrid X Oriental hybrid, AO; and Asiatic hybrid X Lilium longiflorum, AL). Four culture methods¡Xovary slice culture (OSC), ovule with placenta culture (OPC), young single ovule culture (YOC), and embryo (sac) rescue method (ESR)¡Xwere used to culture the fertilized ovules after cut style pollination on plants. The OSC method could be used to obtain hybrid plants in each of the crossing types (0.1-2% success), except for in the OA, AO, and AL crossings. With the OPC method, ovule germinated (0.2-0.8% success) only when Lilium longiflorum "Gerlia" was used as the maternal parent. OSC and OPC required more complicated manipulations. When using the YOC method, ovules were harvested 10 days after the interspecific crosses, and embryo could be germinated from various genotype combinations (0.1-1.1% success). The number of days after pollination (DAP) required for ovule germination in this method (60-125 DAP) was fewer than in the OSC (73-155 DAP) and OPC (57-152 DAP) methods. More plantlets were obtained by the ESR method. The value of the ESR method is that, in the OA crossing, the embryo could only be germinated (0.2-0.3% success) after about 2-3 months of cut style pollination. Apomixes were found in the ovules in some of the cases in this crossing type. No plantlets were obtained in the AL or AO crossings with any of the culture methods tested. Based on the data obtained here, the ESR appears to be the most effective method for obtaining hybrid plants in interspecific hybridization of lily.
KEYWORD	Apomictic embryo; Cut style pollination; Embryo (sac) culture; In vitro culture; Interspecies crosses; Lilium; Ovary slice culture; Ovule with placenta culture; Young single ovule culture;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 43 Number 2 April 2002, page 139-146, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China