TITLE Ultrastructural study of mycoparasitism of Gliocladium roseum on Botrytis cinerea
AUTHOR G.Q. Li
Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta T1J 4B1, Canada
H.C. Huang*
Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta T1J 4B1, Canada
E.G. Kokko
Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta T1J 4B1, Canada
S.N. Acharya
Agriculture and Agri-Food Canada, Lethbridge Research Centre, P.O. Box 3000, Lethbridge, Alberta T1J 4B1, Canada
FULL TEXT [in HTML format] [in PDF format]
ABSTRACT Conidia and germ tubes of Botrytis cinerea were highly susceptible to infection by the mycoparasite Gliocladium roseum, when both organisms were grown as a mixed culture in sterile water containing 8-9×104 pollen grains of alfalfa per ml solution. Scanning electron microscopic studies revealed that infection of conidia and germ tubes occurred through direct penetration by hyphal tips of G. roseum without the formation of appressoria. Transmission electron microscopic studies indicated indentation and rupture of the host cell walls at penetration sites. The parasitized conidia and germ tubes of B. cinerea showed signs of cytoplasmic disintegration and the presence of hyphae of G. roseum.
KEYWORD Alfalfa pollen; Botrytis cinerea; Gliocladium roseum; Mycoparasitism;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 43 Number 3 July 2002, page 211-218, 8 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China