TITLE Photosynthetic potentials of in vitro-grown juvenile, adult, and rejuvenated Sequoia sempervirens (D. Don) Endl. shoots
AUTHOR Li-Chun Huang
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529
Jui-Hsi Weng
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529
Chiu-Hui Wang
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529
Ching-I Kuo
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529
Yuh-Jang Shieh*
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529
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ABSTRACT In vitro shoot tips of Sequoia sempervirens (D. Don.) Endl.ˇXincluding a juvenile, two adult, and two rejuvenated adult clonesˇXwere examined for differences in basic physiological characteristics. Moisture contents were the same, around 85%, for all tissues regardless of origin. Growth rates, determined by fresh weight increase and shoot elongation, were higher for the juvenile and rejuvenated shoots. They correlated with higher total nitrogen contents. Juvenile and rejuvenated shoots also showed higher rates of photosynthesis and respiration, evidenced by faster O2 evolution and consumption. The photosynthetic rates were associated with more chlorophyll, especially chlorophyll a, in the juvenile and the rejuvenated shoots. Nevertheless, identical quantum efficiencies of photosystem II indicated the same photosystems were operating and with equal effectiveness in juvenile, adult, and rejuvenated tissues.
KEYWORD Phase change; Photosynthesis; Respiration; Sequoia sempervirens; Total nitrogen content;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 44 Number 1 January 2003, page 31-35, 5 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China