TITLE	Characterization of Alpha-amylase inhibitor in Vigna sublobata
AUTHOR	E. KOKILADEVI* Center for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India A. MANICKAM Center for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India B. THAYUMANAVAN Center for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India
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ABSTRACT	Alpha-amylase inhibitor protein, which inhibits the activity of insect (Callosobruchus analis) a-amylase, was characterized from V. sublobata. The molecular weight of purified inhibitor protein was 14 kDa by SDS-PAGE. The inhibitor is non-glycosylated protein and its N-terminal sequence is similar (A P S P V…) to Phaseolus vulgaris a-AI-1. Its pI value is 6.0 and largely localised in cotyledons. The inhibitory activity decreased during germination from days one to five. In the developmental stages of seed formation from anthesis to 30 days the inhibitor content increased.
KEYWORD	a-Amylase; Alpha-amylase inhibitor; Callosobruchus analis; Vigna sublobata;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 46 Number 3 July 2005, page 189-196, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China