TITLE	Preparation of amyloplasts from sweet potato callus culture
AUTHOR	Chi-Hui Lu Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China Ping-Du Lee Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China Jong-Ching Su Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China
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ABSTRACT	Culture conditions for sweet potato callus were manipulated to enhance starch content. Tissue cultures were frozen in liquid nitrogen, pulverized in a ceramic mortar, and ground with a buffer and sea sand. Fractional centrifugation in a discontinuous Percoll gradient enabled separation of amyloplasts in a 40% Percoll layer sandwiched between 20% and 50% layers. A latency test of marker enzymes for amyloplast, and a preliminary feeding experiment with radio-labeled substrates for starch synthesis confirmed that the preparation is suitable for use in the study of starch biosynthesis.
KEYWORD	Amyloplast; Callus; Ipomoea batatas; Latency test; Starch metabolism; Sweet potato; Tissue culture;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 36 Number 4 October 1995, page 223-228, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China