TITLE	Polyamines in relation to ammonium-inhibited growth in suspension-cultured rice cells
AUTHOR	Shu Jiuan Chen Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China Ching Huei Kao Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China
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ABSTRACT	The role of endogenous polyamines in affecting the growth inhibition of rice cells fed with ammonium was investigated. When ammonium was used as the sole nitrogen source, the growth of rice cells, as judged by dry or fresh mass, was markedly inhibited. Ammonium-fed rice cells had a higher level of putrescine (Put) than the control cells (supplied with both nitrate and ammonium). However, ammonium-fed cells had much lower levels of spermidine (Spd) and spermine (Spm) than the control. The growth inhibition of rice cells induced by ammonium could not be recovered by the addition of Spd and Spm. D-Arginine or a-methylornithine, inhibitors of Put biosynthesis, caused a reduction in the level of Put in ammonium-fed rice cells, but was unable to reverse the growth inhibition of rice cells fed with ammonium. It was concluded that endogenous polyamines might not play a role in influencing the ammonium-inhibited growth of rice cells.
KEYWORD	Ammonium; Cell growth; Oryza sativa; Putrescine; Spermidine; Spermine;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 37 Number 3 July 1996, page 197-200, 4 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China