TITLE Peroxidase activity in ethylene-, ABA-, or MeJA-treated rice (Oryza sativa L.) roots
AUTHOR Tse-Min Lee
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115, Republic of China
Yaw-Huei Lin
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115, Republic of China
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ABSTRACT Changes of peroxidase (EC 1.11.1.7) activity in ethylene-, ABA- or methyl jasmonate (MeJA)-treated rice (Oryza sativa L. cv. Taichung Native 1) roots were investigated. Guaiacol and syringaldazine were used as substrates for peroxidase activity determination. On a protein or fresh weight basis, activity of wall-bound guaiacol and syringaldazine peroxidase in air-grown roots decreased as growth advanced. In air-grown roots, soluble peroxidase activity on a fresh weight basis also decreased, but activity on a protein basis remained constant. In response to 10 ml/l ethylene, root growth was strongly inhibited while, in contrast, activity of cell wall-bound guaiacol and syringaldazine peroxidase on a protein or fresh weight basis increased. Activity of soluble peroxidase on a fresh weight basis in ethylene-treated roots also increased, although soluble peroxidase activity per mg protein did not change. ABA (10 mM) or MeJA (50 mM) also resulted in an inhibition of root growth but an increase of wall-bound guaiacol and syringaldazine peroxidase activity. Thus, activity of wall-bound peroxidase is inversely related to plant hormone-mediated growth inhibition of rice roots.
KEYWORD Oryza sativa L.; Root; Ethylene; ABA; Methyl jasmonate; Cell wall-bound peroxidase;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 37 Number 3 July 1996, page 201-207, 7 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China