TITLE Identification of dried rhizomes of Coptis species using random amplified polymorphic DNA
AUTHOR Kur-Ta Cheng
Graduate Institute of Pharmacognosy Science, Taipei Medical College, Taipei, Taiwan, Republic of China
Hsien-Chang Chang
National Laboratories of Foods and Drugs, Department of Health Executive Yuan, Taipei, Taiwan, Republic of China
Ching-Hua Su
Center of Biotechnology, Taipei Medical College, Taipei, Taiwan, Republic of China
Feng-Lin Hsu
School of Pharmacy, Taipei Medical College, No. 250, Wu-Hsing Street, Taipei, Taiwan, Republic of China
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ABSTRACT The Random amplified polymorphic DNA (RAPD) technique was adopted in the study to identify eight kinds of Coptidis Rhizomes and one kind of Picrorhizae Rhizoma, a pharmaceutical replacement for the former in some provinces of China. The DNA isolated from the dried rhizome of the samples were used as templates in polymerase chain reactions with twenty random decamer primers. Four of the primers, OPT-09, OPT-15, OPT-18, and OPT-20, revealed reproducibly distinct RAPD profiles. The primers possessed different abilities to detect variations in the Coptis species. RAPD analysis provides an efficient approach to identifying the sources of Coptidis Rhizoma medicines on the market.
KEYWORD Coptidis; Coptis; Fingerprinting; Identification; RAPD;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 38 Number 4 October 1997, page 241-244, 4 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China