TITLE	Phylogenetic position of Raphanus in relation to Brassica species based on 5S rRNA spacer sequence data
AUTHOR	Yau-Wen Yang Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China Pey-Feng Tseng Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China Pon-Yean Tai Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China Cheng-Ju Chang Institute of Biomedical Science, Academia Sinica, Taiwan, Republic of China
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ABSTRACT	Based on RFLP analysis, two evolutionary lineages for Brassica diploid species have been proposed. These are (I) the "nigra" lineage and (II) the "rapa/oleracea" lineage. The phylogenetic relationship of Raphanus species to these two lineages is still unclear because chloroplast and mitochondrial DNA genomic restriction site variation suggests that Raphanus is more closely related to the "rapa/oleracea" lineage, whereas nuclear RFLPs and other lines of evidence suggest that Raphanus belongs to the "nigra" lineage. Here, we present evidence of the intergenic spacer of nuclear 5S rRNA to support that Raphanus is more closely related to the "nigra" lineage than to the "rapa/oleracea" lineage. Genetic polymorphism within species is also discussed.
KEYWORD	Brassica; Phylogeny; Raphanus; 5S rRNA spacer;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 39 Number 3 July 1998, page 153-160, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China