TITLE	Serological characterization of papaya ringspot virus isolates in Taiwan
AUTHOR	Cheng-Ping Kuan National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, 161-2, Kuen Yang Street, Nankang, Taipei 115, Taiwan K.-H. Chen Department of Plant Pathology, National Taiwan University, Taipei 107, Taiwan, Republic of China H. J. Su Department of Plant Pathology, National Taiwan University, Taipei 107, Taiwan, Republic of China
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ABSTRACT	Twenty-seven isolates of papaya ringspot virus (PRSV) were obtained from Taiwan. Differences in symptoms were observed. In addition to such isolates as necrotic severe mottle (SMN), severe mottle (SM) and mild mottle (M), another mottle type with deformation (DF) was isolated. All the isolates of PRSV and PRSV-W (PRSV type W) examined were serologically indistinguishable as determined by polyclonal antibodies-rabbit antisera to PRSV, but were consistently distinguishable with monoclonal antibodies (MAbs). Those isolates were raised against the PRSV. The virus isolates that reacted with the MAbs to PRSV were selected and applied to compare the antigenic specificity of the PRSV strain. Moreover, the immunoreactivities of the MAbs were compared with polyclonal rabbit serum to detect PRSV in foliar tissue by enzyme-linked immunosorbent assay (ELISA). Eleven selected MAbs were differentiated into five sero-groups according to sero-reaction patterns of ELISA index with 27 isolates of PRSV and PRSV-W. It was assumed that at least five antigenic epitopes were identified. Distinct results were obtained in application of MAb to ELISA for detecting PRSV strains in cucurbitaceous plants.
KEYWORD	Papaya ringspot disease; Plant virus; Serology;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 40 Number 3 July 1999, page 231-236, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China