TITLE	Ca2+-dependent excretion of salicylic acid in tobacco cell suspension culture
AUTHOR	Hsien-Jung Chen Postdoctorate, Institute of Botany, Academia Sinica, Nankang, Taipei 11529, Taiwan Joseph Kuc' Professor Emeritus, Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
FULL TEXT	[in HTML format] [in PDF format]
ABSTRACT	The radioactive 14C-Salicylic acid (SA) was used to monitor the metabolism of exogenous SA in tobacco cell suspension culture. Tobacco cells took up ca. 39.46% of the applied SA 5 min after the addition of a final 200 µM SA mixture containing [14C]SA to the cell suspension culture. Most of the SA absorbed by the cells was gradually lost to the culture medium, and 2.62% and 91.55% of the radioactivity were found in the cells and the culture filtrate, respectively, 5 h after treatment. Excretion of SA to the culture medium required external Ca2+ and was inhibited by EGTA pretreatment. The inhibition was reversible through the supplement of Ca2+ to the cell suspension culture. Based on the data, we conclude that SA excretion from the 200 µM-treated cells requires external Ca2+ and likely involves a putative Ca2+-dependent pathway in tobacco suspension culture.
KEYWORD	Ca2+; EGTA; SA loss; Salicylic acid; Tobacco cell suspension culture;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 40 Number 4 October 1999, page 267-273, 7 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China