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TITLE | RAPD analysis of Lycium barbarum medicine in Taiwan market |
AUTHOR | Kur-Ta Cheng Department of Biochemistry, Taipei Medical College, No. 250, Wu-Hsing Street, Taipei 110, Taiwan Hsien-Chang Chang National Laboratories of Foods and Drugs Department of Health Executive Yuan, Taipei, Taiwan Her Huang Department of Anatomy, Taipei Medical College, Taipei, Taiwan Cher-Tong Lin School of Dentistry, Taipei Medical College, Taipei, Taiwan |
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ABSTRACT | In this study, we investigated Lycium barbarum, a Chinese medicine sold on the Taiwan market, using RAPD analysis. Sixty oligonucleotide primers were used to screen twenty randomly selected samples in the analysis. Total DNA extracted from the fruit of the medicine material was used as template in the PCR reaction. Four primers: OPD-15, OPG-15, OPT-12 and OPT-17, showed distinct polymorphic patterns, but others exhibited profiles nearly identical to the other samples used in the study. We found that only two RAPD fingerprinting types of these primers were outlined from twenty collected Lycium samples. Fifteen samples showed the first type of profiles while only five samples resulted of the second type. A low genetic diversity among the Lycium barbarum samples was revealed by RAPD analysis. |
KEYWORD | Chinese medicine; DNA; Lycium barbarum; RAPD; |
ARTICLE INFO | Botanical Bulletin of Academia Sinica, Volume 41 Number 1 January 2000, page 11-14, 4 pages |
PUBLISHER | Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China |