TITLE	A tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. var. gloriosoides Baker
AUTHOR	Chen Chang Institute of Botany, Academia Sinica, Taipei 115, Taiwan Chang-Tesrn Chen Taoyuan District Agricultural Improvement Station, Taoyuan, Taiwan Yu-Ching Tsai Taiwan Seed Improvement and Propagation Station, Hsinshe, Taichung, Taiwan, Wei-Chin Chang Institute of Botany, Academia Sinica, Taipei 115, Taiwan
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ABSTRACT	Floret explant of a local accession of Lilium speciosum Thunb. var. gloriosoides Baker produced subculturable totipotent calli on a Murashige and Skoog basal medium with a supplement of 3 mg/l 2,4-dichlorophenoxyacetic acid and 0.25 mg/l benzyladenine. The calli were able to form bulblets, which subsequently developed into plantlets on the MS basal medium supplemented with 0.1 mg/l naphthalene acetic acid, 1 g/l active charcoal and 170 mg/l NaH2PO4. The rare lily was proliferated in vitro by a scale-bulblet cycling propagation method with a multiplication rate of eight times at three-month intervals. Finally, 6000 plantlets have been produced within nine months. We established 200 plants in the greenhouse under misty conditions for a four-week period with a 98% survival rate. These plants grew well and elongated with normal flowers in the second year.
KEYWORD	Bulblet; Callus; Lilium speciosum Thunb. var. gloriosoides Baker; Rare plant; Scale;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 2 April 2000, page 139-142, 4 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China