TITLE	Sequence and transcript analysis of the orf140-nad3-rps12-atp1 gene cluster in the mitochondrial DNA of mung bean (Vigna radiata L. (Wilzek) cv. Tainan No. 5)
AUTHOR	Yuchang Cheng Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115, ROC Hwa Dai Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115, ROC
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ABSTRACT	An orf140-nad3-rps12-atp1 gene cluster, which spans a 7.1 kb region of the mung bean mitochondrial genome, was identified and completely sequenced. The coding regions of nad3, rps12, and atp1 genes are highly conserved between mung bean and other plant species. The nucleotide sequences of orf140 and its 5'-flanking region are homologous with ORFBs in sunflower and Oenothera, and are also found to be conserved in several chimeric ORFs of various plant CMS lines. The region 3'-downstream from the mung bean atp1 is similar to its counterparts in soybean and common bean, which has been shown to be specific to a subset of legumes. Genes of this cluster are present as a single copy in mung bean. Northern-blot hybridization and RT-PCR suggested that the four genes are co-transcribed and then processed into transcripts of different sizes. Variation of accumulation levels of different transcripts was observed.
KEYWORD	DNA sequence; Mitochondrial gene cluster; Mung bean; orf140-nad3-rps12-atp1; Transcription;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 3 July 2000, page 183-190, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China