TITLE Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae) - an important medicinal herb
AUTHOR Chung-Chuan Chen
Institute of Chinese Pharmaceutical Science, China Medical College, Taichung 40421, Taiwan
Suh-Jau Chen
Institute of Chinese Pharmaceutical Science, China Medical College, Taichung 40421, Taiwan
Abhay P. Sagare
Department of Agronomy, Taiwan Agricultural Research Institute, 189, Chung-Cheng Road, Wu-feng, Taichung 41301, Taiwan
Hsin-Sheng Tsay
Department of Agronomy, Taiwan Agricultural Research Institute, 189, Chung-Cheng Road, Wu-feng, Taichung 41301, Taiwan
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ABSTRACT Efficient plant regeneration system has been established from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae), an important medicinal herb. Adventitious shoots were induced by culturing the stem internode explants on Murashige and Skoog (MS) basal medium supplemented with 2.22-35.51 ÁM N6-benzylaminopurine (BA) in combination with 0.54 ÁM a-naphthaleneacetic acid (NAA). The regeneration potential varied with the developmental stage of the stem explant and growth regulator combination. Adventitious shoots formed a new crop of multiple shoots when subcultured on MS medium supplemented with 17.75 ÁM BA. Shoots were rooted on 1/4-strength MS basal medium supplemented with 5.37 ÁM NAA. Plantlets produced from shoots were transferred to soil and acclimatized in a growth chamber. Plants appearing morphologically normal were obtained six to seven months after culture of stem internode explants.
KEYWORD Adenophora triphylla (Thunb.) A. DC.; Campanulaceae; De novo regeneration; Germplasm conservation; Medicinal herb; Micropropagation; Sha shen;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 42 Number 1 January 2001, page 1-7, 7 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China