TITLE Cold-acclimation and root temperature protection from chilling injury in chilling-sensitive mungbean (Vigna radiata L.) seedlings
AUTHOR Mon-Yaw Chang
Department of Botany, National Taiwan University, Taipei 106, Taiwan, Republic of China
Swu-Ling Chen
Department of Botany, National Taiwan University, Taipei 106, Taiwan, Republic of China
Chung-Fen Lee
Department of Botany, National Taiwan University, Taipei 106, Taiwan, Republic of China
Yih-Ming Chen*
Department of Botany, National Taiwan University, Taipei 106, Taiwan, Republic of China
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ABSTRACT Exposure of mungbean seedlings to 4C for 2 days induced irreversible chilling injury. The major cation in the leakage from tissues of unacclimated seedlings was K+, the loss of which was 7 to 10-fold greater than that of Ca++ or Mg++. Acclimation of seedlings at 10C protected them from the injuries caused by the 4C treatment. Acclimation of seedlings at 10C for 2 to 3 days, significantly decreased the conductivity and the concentration of soluble sugars, free amino acids, and cations (K+, Mg++ and Ca++) in the leakage. Compared to the 28C-root/28C-shoot control seedlings, those in the 28C -root/4C -shoot treatment did not suffer noticeable injury, but seedlings in the 4C-root/4C-shoot treatment did. The solute potential, water potential, and the concentration of free amino acids and cations (K+, Mg++, and Ca++) in the cell sap of the 28C-root/4C-shoot seedlings were similar to those of the control seedlings.
KEYWORD Cell sap; Chilling injury; Cold acclimation; Conductivity; Leakages; Mungbean (Vigna Radiata L.); Root temperature;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 42 Number 1 January 2001, page 53-60, 8 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China