TITLE	Characterization of phytochemicals stimulatory to sexual reproduction in Phytophthora cactorum and P. parasitica
AUTHOR	Hyeong-Jin Jee Department of Plant and Environmental Protection Sciences, Beaumont Agricultural Research Center, University of Hawaii at Manoa, Hilo, Hawaii 96720, USA Chung-Shih Tang Department of Molecular Biosciences and Biosystems Engineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA Wen-Hsiung Ko Department of Plant and Environmental Protection Sciences, Beaumont Agricultural Research Center, University of Hawaii at Manoa, Hilo, Hawaii 96720, USA
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ABSTRACT	Phytophthora cactorum and P. parasitica formed oospores on basal medium supplemented with corn oil or crude soybean lecithin. The non-saponifiable fraction from these two supplements were stimulatory to oospore formation of both fungi, but the saponifiables were stimulatory to only P. cactorum. When the non-saponifiables were subjected to Florisil column chromatography, the fraction eluted with 25% diethyl ether in hexane was the most stimulatory. Thin layer chromatography (TLC) separated this fraction into three active bands. Among them, the two non-digitonide TLC bands were further separated by high performance liquid chromatography (HPLC), and the active fractions were isolated for gas chromatography-mass spectrometry (GC-MS) analysis. Compounds identified were 3-eicosyne, b-farnesene, tetradecane (2, 6, 10-trimethyl), docosane, hexadecanol (2-methyl), phytol, and pentatriacontane. Commercially available phytol tested was found to be highly stimulatory to oospore formation of both fungi. It was active even at the concentration of 1 ng/basal medium disc.
KEYWORD	Corn oil; Phytophthora cactorum; Phytophthora parasitica; Phytol; Sexual reproduction; Soybean lecithin;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 43 Number 3 July 2002, page 203-210, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China