TITLE	Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10% SDS-PAGE gels
AUTHOR	Wen-Chi Hou Graduate Institute of Pharmacognosy Science, Taipei Medical University, Taipei, Taiwan 110 Yeh-Lin Lu School of Pharmacy, Taipei Medical University, Taipei, Taiwan 110 Sin-Yie Liu Taiwan Agricultural Research Institute, Council of Agriculture, Wu-Feng, Taichung, Taiwan 413 Yaw-Huei Lin Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115
FULL TEXT	[in HTML format] [in PDF format]
ABSTRACT	Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx isozymes with molecular masses between 50 and 64 kDa. It was found that SOD isozymes were stable during 40 to 65°C treatment; however, no SOD activity could be detected at either 70 or 80°C for 5 min. In contrast, all GPx isozymes were stable under 80°C for 5 min, and GPx in SL and TL cultivars could resist 80°C treatment for 30 min.
KEYWORD	Glutathione peroxidase; Liriope spicata L.; Superoxide dismutase;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 44 Number 1 January 2003, page 37-41, 5 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China