TITLE Effects of Cryptococcus laurentii (Kufferath) Skinner in combination with sodium bicarbonate on biocontrol of postharvest green mold decay of citrus fruit
AUTHOR Hong-Yin Zhang
Department of Food Science and Nutrition, 2Department of Biology, Zhejiang University, Hangzhou 310029, People's Republic of China
Cheng-Xin Fu

Xiao-Dong Zheng
Department of Food Science and Nutrition, 2Department of Biology, Zhejiang University, Hangzhou 310029, People's Republic of China
Dan He
Department of Food Science and Nutrition, 2Department of Biology, Zhejiang University, Hangzhou 310029, People's Republic of China
Li-Jun Shan
Department of Food Science and Nutrition, 2Department of Biology, Zhejiang University, Hangzhou 310029, People's Republic of China
Xi Zhan
Department of Food Science and Nutrition, 2Department of Biology, Zhejiang University, Hangzhou 310029, People's Republic of China
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ABSTRACT The potential of using Cryptococcus laurentii alone or in combination with sodium bicarbonate solution for control of Penicillium digitatum (green mold) on oranges was investigated. Agar disks of C. laurentii NYDA cultures placed on PDA plates seeded with pathogen did not inhibit the growth of P. digitatum. Spore germination of P. digitatum in PDB was significantly controlled in the presence of living C. laurentii cell suspensions. Cryptococcus laurentii significantly controlled green mold on oranges after challenge with 5 × 104 spores/ml of P. digitatum. The higher the concentrations of the antagonist, the lower the disease incidence regardless of whether the fruit was stored at 20°C for 5 days or 2°C for 50 days. The efficacy of C. laurentii for control of green mold was improved when combined with sodium bicarbonate. The combination of sodium bicarbonate and C. laurentii could be an alternative to fungicides for control of postharvest green mold disease on citrus fruits.
KEYWORD Biocontrol; Citrus fruits; Cryptococcus laurentii; Green mold; Sodium bicarbonate;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 45 Number 2 April 2004, page 159-164, 6 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China