TITLE	Antioxidant activities of methanolic and hot-water extracts from leaves of three cultivars of Mai-Men-Dong (Liriope spicata L.)
AUTHOR	Wen-Chi HOU Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan 110 Wen-Chung WU School of Pharmacy, Taipei Medical University, Taipei, Taiwan 110 Chih-Yuan YANG Llife Science, Liberal Arts Center, HsiWu College, Taipei, Taiwan 224 Hsien-Jung CHEN Department of Horticulture, Chinese Culture University, Taipei, Taiwan 111 Sin-Yie LIU Taiwan Agricultural Research Institute, Council of Agriculture, Wu-Feng, Taichung, Taiwan 413 Yaw-Huei LIN Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115
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ABSTRACT	1,1-dipheny-2-picrylhydrazyl (DPPH) scavenging activities of the 80% methanolic leaf extracts of three cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Mai-Men-Dong (Liriope spicata L.) are analyzed by spectrophotometry. The concentrations required for 50% inhibition (IC50) of DPPH radicals were 81.08, 96.97, and 53.78 mg/mL, respectively. The methanolic extracts were further partitioned into three n-hexane-, ethylacetate-, and water-soluble fractions, among which the ethylacetate-soluble fraction exhibited the highest DPPH scavenging activity. The IC50 of ethylacetate-soluble fractions of SL, BL, and TL for DPPH radical scavenging activity were 41.55, 24.55, and 53.33 mg/mL, respectively. Each Mai-Men-Dong powder (1 g) was deposited in a tea bag and then dipped in hot water (100°C, 100 mL) for 3 min with triplicate samples. These hot-water extracts were then freeze-dried for an anti-DPPH radical capacity test, which found a positive correlation with the phenolic contents of each hot water extract. The IC50 of hot water extracts of SL, BL, and TL for DPPH radical scavenging activities were 378.97, 171.12, and 95.84 mg/mL, respectively. All three hot water extracts can effectively scavenge hydroxyl radical using electron spin resonance (ESR) spectrometry. The IC50 against hydroxyl radical were 80.8, 69.7, and 116 mg/mL, respectively, for the SL, BL, and TL cultivars.
KEYWORD	DPPH radical; Electron spin resonance (ESR); Hydroxyl radical; Hot water extracts; Mai-Men-Dong (Liriope spicata L.); Methanolic extracts;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 45 Number 4 October 2004, page 285-290, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China