TITLE	Cryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification
AUTHOR	Yuan-Long WANG Department of Life Sciences, National Chung-Hsing University, 250, Kuo-Kuang Road, Taichung, Taiwan 40227, ROC Ming-Jen FAN Senior Scientist, National Plant Genetic Resources Center, Taichung, Taiwan, ROC Song-Iuan LIAW Department of Life Sciences, National Chung-Hsing University, 250, Kuo-Kuang Road, Taichung, Taiwan 40227, ROC
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ABSTRACT	Shoot tips from 4- to 6-week-old in vitro grown plantlets of six different cultivars of papaya were cryopreserved by vitrification. Shoot tips were treated in a 2 ml cryo-tube filled with a solution of 2 M glycerol and 0.4 M sucrose at 25°C for 20 min, then dehydrated with 1 ml pre-cooled vitrification solution at 4°C for 60 min. Treated samples were kept in liquid nitrogen for at least one week. The recovery percentages of six cultivars' shoot tips after vitrification were between 48% and 93%. Cryopreserved shoot tips after being warmed resumed growth within 7 days and developed shoots directly without intermediate callus formation. Prolonged storage in the liquid nitrogen up to 2 years had no affect on regeneration. This study has successfully developed a simple and effective protocol for the cryopreservation of papaya shoot tips.
KEYWORD	Carica papaya; Cryopreservation; Papaya; Vitrification;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 46 Number 1 January 2005, page 29-34, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China