TITLE Analysis of 5' region of glutelin genes from wild rice species
AUTHOR Hsin-Kan Wu
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China
Tein-chin Chen
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China
Mei-Chu Chung
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China
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ABSTRACT The structure of the 5' flanking region of glutelin genes amplified from the various wild rice species was analyzed by cloning and sequencing. The results showed that beyond the essential boxes (legumin, CAAT, AACA and TATA), the 5' region of rice glutelin genes have numerous putative enhancers (long-direct and short-direct repeats) and putative regulatory segments (RY repeats, -300 bp elements, nuclear protein binding sites) though portions of a few elements have been deleted in some wild species. The possible roles of most of the putative elements in glutelin gene expression remain to be determined. The sequence length and structure of glutelin 5' regions vary among rice species. On the basis of the length, the degree of homolgy, and the corresponding base substitutions and deletions in the 5' regions of glutelin genes, the authors suggest that glutelin genes, in the subfamily Glua can be classified into three kinds of members, each with its 5' region of 0.5 kb, 0.9 kb, or 1.2 kb. A member gene in the subfamily may reside at one or more loci in a rice genome. The same member gene that appears among rice species with minor deletion, addition, or substitution may be designated as alleles of that gene.
KEYWORD Glutelin gene; 5' Region sturcture; Subfamily GluA; Wild rice;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 37 Number 1 January 1996, page 41-49, 9 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China