 |
|
|
| TITLE | Characterization of the 1.8-kb plasmid pXV64 from Xanthomonas campestris pv. vesicatoria |
| AUTHOR | Shu-Fen Weng Institute of Molecular Biology1, Department of Botany2 and Agricultural Biotechnology Laboratories3, National Chung Hsing University, Taichung 402, Taiwan, Republic of China Nien-Tsung Lin Institute of Molecular Biology1, Department of Botany2 and Agricultural Biotechnology Laboratories3, National Chung Hsing University, Taichung 402, Taiwan, Republic of China Yu-Fen Fan Institute of Molecular Biology1, Department of Botany2 and Agricultural Biotechnology Laboratories3, National Chung Hsing University, Taichung 402, Taiwan, Republic of China Juey-Wen Lin Institute of Molecular Biology1, Department of Botany2 and Agricultural Biotechnology Laboratories3, National Chung Hsing University, Taichung 402, Taiwan, Republic of China Yi-Hsiung Tseng Institute of Molecular Biology1, Department of Botany2 and Agricultural Biotechnology Laboratories3, National Chung Hsing University, Taichung 402, Taiwan, Republic of China |
| FULL TEXT | [in HTML format] [in PDF format] |
| ABSTRACT | A simple rapid plasmid-screening method was developed and then used on 58 strains of Xanthomonas campestris pv. vesicatoria. A very small (1.8-kb) cryptic plasmid, designated pXV64, that had a copy number of about 200 to 350 was detected. A physical map of pXV64 was established and a derivative, designated p64PK, was constructed by cloning a kanamycin resistance cartridge into the unique PstI site of pXV64. p64PK had the ability to transform different strains of Xanthomonas, could be maintained stably, and was compatible with the broad-host-range IncP plasmid pLAFR1. The results of this study suggest that pXV64 has the potential to be developed into vectors for gene cloning in Xanthomonas. |
| KEYWORD | Plasmid; Rapid plasmid-screening method; Vector; Xanthomonas campestris; |
| ARTICLE INFO | Botanical Bulletin of Academia Sinica, Volume 37 Number 2 April 1996, page 93-98, 6 pages |
| PUBLISHER | Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China |
