TITLE Nucleotide sequence of the nifHDK operon in the aerobic nitrogen-fixing unicellular Synechococcus RF-1
AUTHOR Horng-Ming Chen
Department of Biology, National Taiwan Normal University, Taipei, Taiwan 117, Republic of China
Tan-Chi Huang
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 115, Republic of China
Chiu-Yuan Chien
Department of Biology, National Taiwan Normal University, Taipei, Taiwan 117, Republic of China
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ABSTRACT A 5 kb nucleotide sequence which included nifHDK operon was determined. The amino acid sequences deduced from the nifH, nifD, and nifK of Synechococcus RF-1 and from Anabaena 7120 were compared. The start site of transcription was located by primer extension analysis. A region of 405 bp just upstream from the 5' end of nifH showed considerable sequence similarity to the nifU sequences of Anabaena 7120.
KEYWORD Cyanobacteria; nifHDK operon; Nitrogen fixation; Nucleotide sequence; Synechococcus RF-1;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 37 Number 2 April 1996, page 99-105, 7 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China