TITLE	A simple method for obtaining single-spore isolates of fungi
AUTHOR	Wang-Ching Ho Department of Plant Pathology, Beaumont Agricultural Research Center, University of Hawaii at Manoa, Hilo, Hawaii 96720, USA Wen-Hsiung Ko Department of Plant Pathology, Beaumont Agricultural Research Center, University of Hawaii at Manoa, Hilo, Hawaii 96720, USA
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ABSTRACT	A new method of single spore isolation was developed. A 0.1 µl spore suspension was placed on water agar above one of 50_100 circles marked on the bottom of a plate. After 12_24 h, the number of spores in each circle was counted. Single germinating spores in each circle were transferred separately. For all six species of fungi tested, the number of spores in each micro-drop ranged from 0 to 4. More than 50% of the micro-drops contained a single spore with an unbranched germ tube. This method made it easy to locate well separated spores for single-spore isolation, shortened the isolation time by half, and reduced the incubation period from two days to one.
KEYWORD	Single-spore isolation; Zoospores; Conidia;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 38 Number 1 January 1997, page 41-44, 4 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China