TITLE	Identification of ovule-enriched/specific proteins in Cycas taiwaniana using two-dimensional electrophoresis and blotting
AUTHOR	Jia-Hwey Hu Agricultural Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan, Republic of China Jia-Yu Huang Agricultural Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan, Republic of China Co-Shine Wang Agricultural Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan, Republic of China
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ABSTRACT	We utilized two-dimensional polyacrylamide gel electrophoresis, coupled with silver stain, to identify five ovule-enriched and three ovule-specific polypeptides in Cycas taiwaniana Carr. (CTO). These ovule-enriched and -specific proteins are all with acidic pIs in the 4.8_6.4 range. Polyclonal antiserum to an ovule polypeptide (CTO-22) was prepared and affinity-purified to investigate the specificity of the protein. Immunoblot analyses of total protein from the vegetative organs and from organs of the strobili demonstrated that the CTO-22 polypeptide was ovule-specific. The amino terminal sequence of the CTO-22 protein exhibits no similarity with any known protein. To our knowledge, this is the first ovule-specific protein reported in gymnosperms.
KEYWORD	Cycas taiwaniana; 2D PAGE; Immunoblotting; Ovule-enriched/specific proteins;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 38 Number 2 April 1997, page 109-114, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China