TITLE	Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean
AUTHOR	Ching-Yu Lin Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China Cheng-Chun Kuan Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China Rong-Huay Juang Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China Samson C. S. Tsou Asian Vegetable Research and Development Center, P.O. Box 42, Shanhua, Tainan 741, Taiwan, Republic of China C. George Kuo Asian Vegetable Research and Development Center, P.O. Box 42, Shanhua, Tainan 741, Taiwan, Republic of China Ching-San Chen Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China
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ABSTRACT	We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1_1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.
KEYWORD	Differentially expressed cDNAs; Mungbean; Near isogenic line;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 39 Number 2 April 1998, page 87-91, 5 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China