TITLE	Daylength affects protein pattern and flowering in tuberose (Polianthes tuberose L.)
AUTHOR	Yu-Pei Chang Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung City, Taiwan, Republic of China Shih-Fen Ding Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung City, Taiwan, Republic of China Chin-Chih Chou Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung City, Taiwan, Republic of China Bo-Shiun Du Department of Horticulture, National Chia-Yi Institute of Technology, Chia-Yi City, Taiwan, Republic of China Wen-Shaw Chen Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung City, Taiwan, Republic of China
FULL TEXT	[in HTML format] [in PDF format]
ABSTRACT	Tuberose (Polianthes tuberose L. cv. Double) was exposed to 8 h and 16 h daylengths, to investigate the effects of these conditions on floral initiation and development. Corm apices remained vegetative when plant height was 0_5 cm at 8 h daylength. Floral initiation was attained at five-thirteenth (38%) of this stage at 16 h daylength. However, no significant difference in floral initiation or development of tuberose was found between 8 and 16 h daylengths when plants were over 5 cm in height. When tuberose seedlings were exposed to 16 h daylength, differences in protein pattern of the corm tissues were observed at 19, 23, 27, and 57 kDa. Furthermore, a significant protein band at 11 kDa was found in 16 h daylength but not in 8 h daylength, when plant heights were 6 to 30 cm. However, no significant difference in protein patterns was found between daylengths when plant heights were over 30 cm. The electrophoregrams obtained by two-dimensional electrophoresis allowed characterization of about 185 protein spots in corm tissues when plants were 0_5 cm in height and exposed to 8 and 16 h daylengths. At this stage, 20 newly synthesized proteins were observed in 16 h daylength, and 4 other polypeptides showed significant increase in 16 h, as compared to 8 h, daylength. The most significant peptides were 57 kDa protein with isoelectric point 5.1. These results are discussed in connection to flowering.
KEYWORD	Daylength; Flowering; Polianthes tuberose; Polypeptide;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 39 Number 3 July 1998, page 199-203, 5 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China