TITLE	Cytostructure, lipopolysaccharides, and cell proteins analysis from Rhizobium fredii
AUTHOR	Fang-Lin Yang Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan, Republic of China Liang-Ping Lin Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan, Republic of China
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ABSTRACT	The symbiotic roles of rhizobia with legumes have been extensively investigated for agricultural applications. We isolated several strains of rhizobia from Taiwanese soils and analyzed their cellular proteins and lipopolysaccharide (LPS) profiles as well as their ultrastructure by electron microscopy. It was found that the flagella have different forms and locations on the surface of fast-growing and slow-growing rhizobia. In addition, the results of their ultrastructural visualization confirmed that poly-b-hydroxybutyrate (PHB) and polyphosphates are present in rhizobia. The ladder profiles of LPS and cellular proteins on sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that different strains of rhizobia possess different profiles, but strains which belong to the same species have similar profiles. It was also shown by Western blot and immunoassay that strain specificity exists between rhizobia cellular proteins and the antisera of anti-rhizobia cells. These results confirm that indigenous R. fredii exhibits a significant difference from other species of Rhizobiaceae.
KEYWORD	Anti-cellular protein antibody; Anti-LPS antibody; Cell proteins; Cytostructure; Lipopolysaccharides; Rhizobium fredii; Soybean;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 39 Number 4 October 1998, page 261-267, 7 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China