TITLE	Glycine formosana Hosokawa in Taiwan: pod morphology, allozyme, and DNA polymorphism
AUTHOR	F.S. Thseng Department of Agronomy, National Chung Hsing University, Taichung 402, Taiwan F.S. Thseng Department of Agronomy, National Chung Hsing University, Taichung 402, Taiwan S.J. Tsai Department of Agronomy, National Chung Hsing University, Taichung 402, Taiwan J. Abe Laboratory of Plant Genetic Resources, Hokkaido University, Japan S.T. Wu Department of Agronomy, National Chung Hsing University, Taichung 402, Taiwan
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ABSTRACT	Glycine formosana Hosokawa is distributed over the grassland along the riverside and roadside at Dahshi, Guanshi, and Herngshan in Taoyuan county, Taiwan. It is a twining annual herb. It was long considered to be of the same species as G. soja, distributed widely in East Asia. Because the plant showed a continuous variation with G. soja in appearance, Tateishi and Ohashi (1992) considered it a geographic subspecies and renamed it G. max subsp. formosana (Hosokawa) Tateishi et Ohashi. This study attempts to determine the differences between G. formosana and the G. soja collected in China, Korea, and Japan. The materials were planted at National Chung-Hsing University. The pods, allozymes, and DNA polymorphisms were investigated. Glycine formosana has small seeds which are significantly different from the G. soja in China, Korea, and Japan. Lap1-d ¡X one of the 16 loci of 9 allozymes exists only in G. formosana. Twenty-five random sequence 10-mer primers were employed in RAPD analysis for all samples. Twenty-one produced bands, and 14 of those showed polymorphisms. One hundred and thirty-two bands were produced, and 84 bands (64%) showed polymorphisms. Based on the appearances of markers, the genetic similarity coefficients were calculated. Among different samples of G. formosana, few genetic variations were observed (0.885~0.887). However, G. formosana showed marked differences from G. soja in China and Korea and nested within the Japan accessions.
KEYWORD	Allozyme; DNA polymorphism; Glycine formosana; G. soja; Pod; Seed;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 40 Number 4 October 1999, page 251-257, 7 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China