TITLE	Promoter activity of a soybean gene encoding a seed maturation protein, GmPM9
AUTHOR	Pei-Fang Lee Graduate Institute of Botany, National Taiwan University, Taipei, Taiwan, Republic of China Yue-Ie C. Hsing Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China Teh-Yuan Chow Graduate Institute of Botany, National Taiwan University, Taipei, Taiwan, Republic of China Teh-Yuan Chow Graduate Institute of Botany, National Taiwan University, Taipei, Taiwan, Republic of China Teh-Yuan Chow Graduate Institute of Botany, National Taiwan University, Taipei, Taiwan, Republic of China
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ABSTRACT	Late embryogenesis abundant (LEA) proteins are synthesized during the late stages of seed development and have been widely reported in monocot and dicot plants. In order to understand the developmental regulation of the promoter of a soybean gene encoding LEA protein GmPM9, we fused a series of fragments of the promoter region to the b-glucuronidase (GUS) reporter gene (uirA) and transformed the resulting constructs (pZP966, pZP572, pZP510, pZP294 and pZP114) into Arabidopsis thaliana. GUS enzyme activities were detected in mature seeds and cotyledons and hypocotyls of seedlings in transgenic plants containing any one of the five constructs; they were not detected in other tissues at different developmental stages. During seed development, GUS began to appear at 10 days after flowering (DAF) and increased rapidly to a maximum in the mature seeds at 14 DAF. The longest promoter construct (pZP966) enabled the transgenic plants to exhibit the highest GUS activity, whereas the shortest construct (pZP114) was sufficient to direct the expression of the GUS gene at a detectable level. The above findings indicate that the promoter of gGmPM9 can be used to express desirable genes in seeds during late seed maturation. The expression of the GUS gene could be induced in the leaves of the transgenic plants containing pZP966 by salt and desiccation, but not by ABA treatment.
KEYWORD	b-glucuronidase; Late embryogenesis abundant; Promoter; Transgenic Arabidopsis;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 3 July 2000, page 175-182, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China