TITLE	Dioscorins from different Dioscorea species all exhibit both carbonic anhydrase and trypsin inhibitor activities
AUTHOR	Wen-Chi Hou Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China Hsien-Jung Chen Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China Yaw-Huei Lin Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China
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ABSTRACT	Dioscorins, the storage proteins of yam tubers, from six cultivars of three Dioscorea species including D. batatas, D. alata, and D. pseudojaponica were detected in crude extracts of yam tubers but not leaves by immuno stainings on PVDF membrane with a polyclonal antibody which was raised against 28 kDa dioscorin from Dioscorea batatas Decne. It was demonstrated that the dioscorins from the above-mentioned yam species exhibited both carbonic anhydrase (CA) and trypsin inhibitor activities by activity stainings on 15% SDS-PAGE gels. Dioscorins were identified as glycoproteins by ConA-peroxidase activity staining. Dioscorins from D. batatas Decne contained no zinc atoms detectable by atomic absorption spectrophotometry. Neither 5 mM acetazolamide nor 2,6-pyridinedicarboxylic acid inhibited the CA activity of dioscorins.
KEYWORD	Acetazolamide; Carbonic anhydrase; Dioscorin; Pyridinedicarboxylic acid; Storage proteins; Trypsin inhibitor; Yam;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 3 July 2000, page 191-196, 6 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China