TITLE	An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers
AUTHOR	Jou-Ann Lai Department of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China Wei-Chen Yang Department of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China Ju-Ying Hsiao* Department of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China
FULL TEXT	[in HTML format] [in PDF format]
ABSTRACT	Tea (Camellia sinensis [L.] O. Kuntze) is an important beverage crop in Taiwan. Most of the cultivated clones were introduced from China and India though some native wild teas are distributed in the mountains of central and southern Taiwan. In this study, 37 tea samples were evaluated using RAPD and ISSR markers. The samples comprised 21 clones of China, 3 clones of Assam, 7 hybrid clones between China and Assam tea, and 6 individual samples of native Taiwanese wild tea. A total of 53 and 56 polymorphic RAPD and ISSR markers respectively, were scored. The results of cluster analysis based on RAPDs revealed that three major groups could be recognized, i.e., cultivars of China tea and the cultivars developed in Taiwan from hybridization and selection; Assam tea; native Taiwanese wild tea. The native Taiwanese wild teas were, however, most distant in the clustering tree. In the ISSR dendrogram, Taiwanese native wild teas clustered closely with Assam tea then with China tea and the Taiwanese hybrid cultivars. The population gene diversity of the native wild tea was found to be the highest among the three populations studied. Analysis of molecular variance (AMOVA) revealed that the variance component within groups was larger than that among groups. The correlation coefficient between similarity matrices based on RAPD and ISSR was 0.811. A Mantel test revealed that the correlation was highly significant (p<0.001), indicating good congruence between the results of these two molecular markers.
KEYWORD	AMOVA; Camellia sinensis; Genetic variation; ISSR; RAPD; Tea;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 42 Number 2 April 2001, page 93-100, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China