TITLE	Characterization of a 10 cM region of rice chromosome 5
AUTHOR	Teh-Yuan Chow Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China Ya-Ting Chao Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China Su-Mei Liu Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China Hong-Pang Wu Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China Mu-Kuei Chu Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China Ching-San Chen Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China Yue-Ie C. Hsing* Institute of Botany, Academia Sinica, Taipei 115, Taiwan, Republic of China
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ABSTRACT	Rice is a model species for the cereals and a good candidate for genome sequencing due to its relatively small genome (430 Mb), dense physical and genetic maps, and good transgenic systems. As part of an international effort to decode the rice genome, a PAC clone localized at 10 cM of chromosome 5 is completely determined for its sequence using shotgun libraries of its two inserts, 2-kb and 5-kb in length. In total 2,998 sequencing reads were used for the assembly of the final sequence, covering 175,439 bp. This sequence may code for at least 28 putative proteins, as deduced from computational search for homology with other known coding sequences and EST, or predicted using GenScan package. Also present in this sequence are simple repeats, palindrome and retrotransposons. On the basis of these findings, the gene density in the gene-rich region of rice genome is about 6 kb/gene.
KEYWORD	Annotation; High-throughput genome sequencing; Repetitive sequences; Retrotransposons; Rice genome;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 42 Number 3 July 2001, page 159-166, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China